Alturki began his career in New York City at Arthur Andersen, where he worked with the management consulting group for three years after obtaining a Bachelor's degree in Economics from Williams College, Williamstown, Massachusetts, in the United States in 1995.
 
After moving back home in 2000, Rami moved across companies within Alturki Group, building knowledge and experience along the way, until being appointed as President & Chief Executive Officer of  Khalid  Ali Alturki & Sons Holding Co. (Alturki Holding), and serves as Chairman of the boards of its subsidiary companies operating in diverse sectors including construction, infrastructure, industrial services and energy, security technologies, and transportation services. Additionally, he holds different positions on the boards of Alturki’s joint ventures, including Chairman of the board for Velosi Saudi Arabia and Chairman of Element Saudi Arabia, Managing Director of Nesma & Partners, alongside his role as Chairman of the Executive Committee of the Board of Directors.
 
Alturki is an active figure in various fields of investment. During his career, he founded Alturki Industrial Investments and Alturki Ventures to support the group's diversification into industrial and advanced technological investments. He also established Adeem, for real estate development and management.
 
Outside the group, Alturki is active on many committees and councils in the Kingdom of Saudi Arabia. He represents the private sector as a member of the board of the Saudi Authority for Industrial Cities and Technology Zones (MODON), an independent member of the board of the King Fahad Causeway Authority on the Saudi side, a member of the board of the Industrial Advisory Council, College of Design and Built Environment at King Fahd University of Petroleum and Minerals (KFUPM), and a member of the board of the US-Saudi Arabian Business Council. Additionally, he was previously a member of the Saudi Standards, Metrology and Quality Organization (SASO).
 
Alturki is passionate about giving back to his community and actively supports many leading non-profit organizations. He is a member of the Advisory Board of Dhahran Ahliyya Schools, a non-profit school founded by his family and dedicated to delivering the highest quality education as well as making a positive contribution to the sector across the Arab world. He is also a founder and Chairman of Shabab Mujtamie, a social entrepreneurship enterprise dedicated to improving the productivity of Saudi youth by training and developing youth workers. Additionally, he is a founding member and board member of the Ta'allum Association, which supports education and serves on the board of the Prince Sultan bin Abdulaziz Fund for Women’s Development. His involvement in the educational sector extends to founding the Saudi Office of Education for Employment, which is an international non-governmental, non-profit organization aiming to promote sustainable development for Arab youth.
 
He is a founding member and board member of Endeavor Saudi Arabia, a non-profit organization that supports high-impact entrepreneurs. His commitment to youth development extends to sports, where he has been appointed as a board member of Al-Qadsiah Club. Expanding his activities to the health sector, he serves on the board of the Disabled Association in the Eastern Province and is the founding Chairman of the Society of Craniofacial & Cleft Patients.
 
His commitment to community service also extends to Athath Cooperative Association, where he is a founding member. Additionally, he has previously served as a member of the Saudi Food Bank.